Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter (Latris lineata)
Identifieur interne : 000C64 ( Main/Exploration ); précédent : 000C63; suivant : 000C65Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter (Latris lineata)
Auteurs : A. J. Ritar [Australie] ; M. Campet [Australie, Viêt Nam]Source :
- Theriogenology [ 0093-691X ] ; 2000.
English descriptors
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Abstract
Methods of short-term storage and cryopreservation were examined for semen from striped trumpeter (Latris lineata). For fresh semen at 18°C, the percentage of motile sperm declined rapidly from over 80% immediately after activation with sea water to less than 2% within 9 mm after activation The motility after activation of undiluted fresh sperm stored at 5°C was maintained for two days and then declined markedly so that by the eighth day, sperm were mostly immotile after activation The post-thawing motility was higher for sperm frozen with a non-activating diluent containing 2 84 M DMSO in saline (117 mM NaCl) than in an activating glycerol (2 M) medium in dilute sea water (300 mOsm). Post-thawing motility was higher for a dilution rate of 1:5 (semen:diluent) than 1 2 or 1.11 but was similar when frozen semen was thawed at 10 °, 20 ° or 30 °C For semen stored at a range of volumes as pellets frozen on dry ice (0.2 to 2 0 mL) or straws frozen in liquid nitrogen vapor (0 25 to 0 5 mL) and thawed in a waterbath at 20 °C, the post-thawing motilities were similar even though the patterns of cooling and thawing differed markedly between methods of freezing and sizes of pellets and straws
Url:
DOI: 10.1016/S0093-691X(00)00363-0
Affiliations:
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J." last="Ritar">A. J. Ritar</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Tasmanian Aquaculture and Fisheries Institute, University of Tasmania Hobart, Tasmania, 7001</wicri:regionArea><wicri:noRegion>7001</wicri:noRegion></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Australie</country></affiliation></author><author><name sortKey="Campet, M" sort="Campet, M" uniqKey="Campet M" first="M" last="Campet">M. 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For fresh semen at 18°C, the percentage of motile sperm declined rapidly from over 80% immediately after activation with sea water to less than 2% within 9 mm after activation The motility after activation of undiluted fresh sperm stored at 5°C was maintained for two days and then declined markedly so that by the eighth day, sperm were mostly immotile after activation The post-thawing motility was higher for sperm frozen with a non-activating diluent containing 2 84 M DMSO in saline (117 mM NaCl) than in an activating glycerol (2 M) medium in dilute sea water (300 mOsm). Post-thawing motility was higher for a dilution rate of 1:5 (semen:diluent) than 1 2 or 1.11 but was similar when frozen semen was thawed at 10 °, 20 ° or 30 °C For semen stored at a range of volumes as pellets frozen on dry ice (0.2 to 2 0 mL) or straws frozen in liquid nitrogen vapor (0 25 to 0 5 mL) and thawed in a waterbath at 20 °C, the post-thawing motilities were similar even though the patterns of cooling and thawing differed markedly between methods of freezing and sizes of pellets and straws</div></front></TEI><affiliations><list><country><li>Australie</li><li>Viêt Nam</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Ritar, A J" sort="Ritar, A J" uniqKey="Ritar A" first="A. J." last="Ritar">A. J. Ritar</name></noRegion><name sortKey="Campet, M" sort="Campet, M" uniqKey="Campet M" first="M" last="Campet">M. Campet</name><name sortKey="Ritar, A J" sort="Ritar, A J" uniqKey="Ritar A" first="A. J." last="Ritar">A. J. Ritar</name></country><country name="Viêt Nam"><noRegion><name sortKey="Campet, M" sort="Campet, M" uniqKey="Campet M" first="M" last="Campet">M. Campet</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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